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recombinant mouse α klotho  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse α klotho
    Recombinant Mouse α Klotho, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+%CE%B1-klotho/Recombinant+Mouse+Klotho+(aa+35-982)+Protein%2C+CF/10__1002_slash_rco2__70016-111-0-6
    Average 94 stars, based on 56 article reviews
    recombinant mouse α klotho - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.

    Inhibition:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.

    Injection:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.

    Immunoprecipitation:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.

    Incubation:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.

    Quantitation Assay:

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In high-fat diet–induced obesity (DIO) mice, central administration of either 2.0 μg recombinant α-klotho (R&D Systems) alone, 25.0 μg PD173074 alone, 25.0 μg PD173074 10 min before 2.0 μg α-klotho , or vehicle was performed daily.

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging.
    Article Snippet: K. Wang,[+] H. Iijima, F. Ambrosio[+] Department of Physical Medicine and Rehabilitation University of Pittsburgh Pittsburgh, PA 15213, USA E-mail: kwang36@mgh.harvard.edu; fambrosio@mgh.harvard.edu K. Wang, Z. R. Hettinger, F. Ambrosio Discovery Center for Musculoskeletal Recovery Schoen Adams Research Institute at Spaulding Charlestown, MA 02129, USA K. Wang, Z. R. Hettinger, F. Ambrosio Department of Physical Medicine and Rehabilitation Harvard Medical School Boston, MA 02115, USA S. H. Smith, A. Mallepally, S. G. Shroff, F. Ambrosio Department of Bioengineering University of Pittsburgh Pittsburgh, PA 15213, USA Z. R. Hettinger Department of Medicine Division of Geriatric Medicine University of Pittsburgh Pittsburgh, PA 15213, USA S. G. Shroff, F. Ambrosio McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA 15213, USA

    Article Title: α-Klotho protects against oxidative damage in pulmonary epithelia
    Article Snippet: Quantitation of α-Klotho Soluble α-Klotho in rodent plasma was determined by immunoprecipitation-immunoblot assay using recombinant α-Klotho (R&D Systems, Minneapolis, MN) as calibration standard curve. α-Klotho from 100 μl of rat serum plasma was incubated with 2 μg/ml of synthetic sb106 Fabs to α-Klotho.

    Article Title: Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice
    Article Snippet: In DIO mice, ICV administration of either 2.0 μg recombinant α-klotho (R&D Systems), 10 ng wortmannin, 10 ng wortmannin 1 h before 2.0 μg α-klotho , or vehicle was performed the night before a GTT or ITT.



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    Fig. 1 | <t>Klotho</t> induces platelet activation in the blood and increases circulating platelet factors. a, Paradigm for plasma proteomics profiling. Young mice (male, age 4 months) were treated with either vehicle (Veh) (n = 10 mice) or klotho (n = 9 mice) (s.c., 10 μg kg−1) followed by plasma proteomics analysis. b, Percentage of alternations among arms during exploration of the Y maze. Young mice (male, age 4 months, n = 9 mice per group) were treated with either Veh or klotho. *P = 0.014 (two-tailed t-test). c, Plasma proteomics by mass spectrometry analysis 4 h after treatment with Veh or klotho identified six differentially expressed proteins (Q < 0.05, dashed horizontal line; and fold change >2, dashed vertical line). d, Canonical functions of top six differentially expressed plasma proteins following klotho treatment (Q < 0.05 and fold change >2). e, Paradigm for measuring platelet activation. Young mice (male, age
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    Image Search Results


    Fig. 1 | Klotho induces platelet activation in the blood and increases circulating platelet factors. a, Paradigm for plasma proteomics profiling. Young mice (male, age 4 months) were treated with either vehicle (Veh) (n = 10 mice) or klotho (n = 9 mice) (s.c., 10 μg kg−1) followed by plasma proteomics analysis. b, Percentage of alternations among arms during exploration of the Y maze. Young mice (male, age 4 months, n = 9 mice per group) were treated with either Veh or klotho. *P = 0.014 (two-tailed t-test). c, Plasma proteomics by mass spectrometry analysis 4 h after treatment with Veh or klotho identified six differentially expressed proteins (Q < 0.05, dashed horizontal line; and fold change >2, dashed vertical line). d, Canonical functions of top six differentially expressed plasma proteins following klotho treatment (Q < 0.05 and fold change >2). e, Paradigm for measuring platelet activation. Young mice (male, age

    Journal: Nature aging

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.

    doi: 10.1038/s43587-023-00468-0

    Figure Lengend Snippet: Fig. 1 | Klotho induces platelet activation in the blood and increases circulating platelet factors. a, Paradigm for plasma proteomics profiling. Young mice (male, age 4 months) were treated with either vehicle (Veh) (n = 10 mice) or klotho (n = 9 mice) (s.c., 10 μg kg−1) followed by plasma proteomics analysis. b, Percentage of alternations among arms during exploration of the Y maze. Young mice (male, age 4 months, n = 9 mice per group) were treated with either Veh or klotho. *P = 0.014 (two-tailed t-test). c, Plasma proteomics by mass spectrometry analysis 4 h after treatment with Veh or klotho identified six differentially expressed proteins (Q < 0.05, dashed horizontal line; and fold change >2, dashed vertical line). d, Canonical functions of top six differentially expressed plasma proteins following klotho treatment (Q < 0.05 and fold change >2). e, Paradigm for measuring platelet activation. Young mice (male, age

    Article Snippet: Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g−1 (adjusted to weight of mouse) at a dose of 10 μg kg−1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g−1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg−1.

    Techniques: Activation Assay, Clinical Proteomics, Two Tailed Test, Mass Spectrometry